4XAQ
mGluR2 ECD and mGluR3 ECD with ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-17 |
| Detector | RAYONIX MX-225 |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 81.644, 159.652, 93.880 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.520 - 2.210 |
| R-factor | 0.1877 |
| Rwork | 0.186 |
| R-free | 0.22470 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (BUSTER 2.11.5) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.520 |
| High resolution limit [Å] | 2.210 |
| Number of reflections | 61903 |
| <I/σ(I)> | 8.6 |
| Completeness [%] | 99.0 |
| Redundancy | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | 100mM MES pH 6 + 25% PEG 4K + 200mM Ammonium Sulfate |
