4X9J
EGR-1 with Doubly Methylated DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2014-11-02 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 44.009, 55.989, 128.952 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 27.357 - 1.412 |
| R-factor | 0.1738 |
| Rwork | 0.172 |
| R-free | 0.20020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4r2a |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.338 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: dev_1810)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 43.000 | 43.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 3.800 | 1.400 |
| Rmerge | 0.127 | 0.061 | 0.653 |
| Rmeas | 0.131 | 0.063 | 0.719 |
| Rpim | 0.036 | 0.015 | 0.292 |
| Total number of observations | 447598 | ||
| Number of reflections | 29071 | ||
| <I/σ(I)> | 10.7 | ||
| Completeness [%] | 90.6 | 97.2 | 46 |
| Redundancy | 15.4 | 16.7 | 5.7 |
| CC(1/2) | 0.995 | 0.671 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 25mM BisTris pH8, 10% PEG-600, 450mM NaCl |
