4X9C
1.4A crystal structure of Hfq from Methanococcus jannaschii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.86 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.667, 66.857, 109.212 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.667 - 1.400 |
| R-factor | 0.1673 |
| Rwork | 0.166 |
| R-free | 0.18770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2qtx |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.070 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.450 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.051 | 0.661 |
| Number of reflections | 83538 | |
| <I/σ(I)> | 21.8 | 2.85 |
| Completeness [%] | 99.7 | 97.3 |
| Redundancy | 6.5 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 296 | A2 JBCScreen Nuc-Pro 1 (50% PEG200, 0,1M Tris-HCl, pH 8,0) |
