4X5V
Crystal structure of the post-catalytic nick complex of DNA polymerase lambda with a templating A and incorporated 8-oxo-dGMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-07-18 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.010, 62.842, 141.507 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.985 - 2.150 |
| R-factor | 0.2166 |
| Rwork | 0.214 |
| R-free | 0.24500 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.958 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.985 | 2.157 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.045 | 0.800 |
| Number of reflections | 27980 | |
| <I/σ(I)> | 26.4 | 2.7 |
| Completeness [%] | 99.0 | 99 |
| Redundancy | 8.7 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.1 M sodium citrate pH 5.6 and 1 M ammonium phosphate monobasic |
