4X57
Structure of an Arabidopsis E2 / Membrane-anchored Ubiquitin-fold Protein Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-04-20 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97934 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 135.716, 135.716, 202.134 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 10.000 - 2.800 |
R-factor | 0.22301 |
Rwork | 0.221 |
R-free | 0.25682 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | ID 3NOB 1qcq |
RMSD bond length | 0.010 |
RMSD bond angle | 1.544 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.070 | 1.000 |
Number of reflections | 27721 | |
<I/σ(I)> | 30 | 1.95 |
Completeness [%] | 99.8 | 100 |
Redundancy | 7.2 | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 280 | 3.0 M (NH4)2SO4 |