4X51
X-ray structure of mouse interleukin-10 mutant - S1_E8del, C149Y
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-12 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.91841 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 39.770, 80.820, 84.320 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.150 - 2.050 |
| R-factor | 0.1911 |
| Rwork | 0.188 |
| R-free | 0.24632 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lk3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.548 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.150 | 2.160 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.083 | 0.556 |
| Number of reflections | 17734 | |
| <I/σ(I)> | 12.3 | 2.9 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 4.8 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | Crystals grew at 20-30% (v/v) glycerolethoxylate, 0.2M ammonium acetate, 0.1M MES (pH 6.3 - 6.8). Crystals appeared after 1-2 days and reached their final size after 3-4 days. |
