4X4W
Crystal structure of the full-length human mitochondrial CCA-adding enzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-12-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.970, 142.360, 100.590 |
| Unit cell angles | 90.00, 94.64, 90.00 |
Refinement procedure
| Resolution | 29.076 - 1.900 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.19580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.572 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 1.950 | ||
| High resolution limit [Å] | 1.900 | 8.500 | 1.900 |
| Rmerge | 0.049 | 0.016 | 0.536 |
| Rmeas | 0.059 | 0.019 | 0.650 |
| Total number of observations | 271414 | ||
| Number of reflections | 89161 | 994 | 6668 |
| <I/σ(I)> | 15.69 | 51.55 | 2.33 |
| Completeness [%] | 98.9 | 95.3 | 99.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 2.2M Ammonium Sulfate, 0.05M Na Citrate pH 5.5 |
