4X4O
Crystal structure of the A.fulgidus CCA-adding enzyme in complex with a G70A arginyl-tRNA minihelix and CTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-10-03 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 111.059, 215.602, 58.629 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.680 - 3.201 |
R-factor | 0.2116 |
Rwork | 0.209 |
R-free | 0.25110 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.755 |
Data reduction software | XDS |
Data scaling software | SCALA (3.3.20) |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.791 | 29.791 | 3.370 |
High resolution limit [Å] | 3.201 | 10.120 | 3.200 |
Rmerge | 0.043 | 0.483 | |
Rmeas | 0.139 | ||
Rpim | 0.075 | 0.030 | 0.313 |
Total number of observations | 75872 | 2352 | 10887 |
Number of reflections | 23753 | ||
<I/σ(I)> | 8.4 | 21 | 2.5 |
Completeness [%] | 99.2 | 95.2 | 98.7 |
Redundancy | 3.2 | 2.9 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 290 | 18.6% PEG 3,350, 0.2M potassium sodium tartrate, 0.1M Sodium Acetate pH 5.6 |