4X3K
Crystal structure of chromobox homolog 7 (CBX7) chromodomain with H3K27me3 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-04 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.460, 33.230, 46.160 |
Unit cell angles | 90.00, 95.80, 90.00 |
Refinement procedure
Resolution | 30.710 - 1.450 |
R-factor | 0.19089 |
Rwork | 0.189 |
R-free | 0.23213 |
RMSD bond length | 0.023 |
RMSD bond angle | 2.346 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | BALBES |
Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.710 | 1.520 |
High resolution limit [Å] | 1.450 | 1.450 |
Number of reflections | 24687 | |
<I/σ(I)> | 18.3 | 5.8 |
Completeness [%] | 99.4 | 99 |
Redundancy | 7.2 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | 25% PEG MME550, 0.1 M MES pH6.5, 0.01 M zinc sulfate |