4WYM

Structural basis of HIV-1 capsid recognition by CPSF6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-07-02
Detector	PSI PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	134.790, 136.030, 207.257
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.468 - 2.600
R-factor	0.2194
Rwork	0.219
R-free	0.25920
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	3h4e
RMSD bond length	0.003
RMSD bond angle	0.564
Data reduction software	XDS
Data scaling software	XDS
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.470	2.740
High resolution limit [Å]	2.600	2.600
Rmerge		0.863
Number of reflections	117525
<I/σ(I)>	20.2	2.3
Completeness [%]	100.0	100
Redundancy	6.8	7.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	0.1 M sodium formate, ammonium acetate, tri-sodium citrate, sodium/potassium tartrate, sodium oxamate; 0.1 M sodium HEPES, MOPS; 30% glycerol, PEG 4000