4WYL
Mutant K18E of 3D polymerase from Foot-and-Moth Disease Virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-04-12 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1.4 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 91.627, 91.627, 117.852 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.810 - 2.000 |
| R-factor | 0.21498 |
| Rwork | 0.214 |
| R-free | 0.24258 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.807 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.8.0069) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.810 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.061 | 0.350 |
| Number of reflections | 34625 | |
| <I/σ(I)> | 25.6 | 5.3 |
| Completeness [%] | 99.9 | 99.3 |
| Redundancy | 6.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 30% PEG 4K, 0.2M Mg(CH3COO)2, 0.1M MES ph 6.0, 4% gamma-butyrolactone |
