4WY7
Crystal structure of recombinant 4E10 expressed in Escherichia coli with epitope bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 157.300, 44.580, 85.400 |
| Unit cell angles | 90.00, 113.80, 90.00 |
Refinement procedure
| Resolution | 44.700 - 2.100 |
| R-factor | 0.16397 |
| Rwork | 0.162 |
| R-free | 0.20720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fx7 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.526 |
| Data reduction software | MOSFLM (7.1.0) |
| Data scaling software | SCALA (3.3.21) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 2.210 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.102 | 0.370 |
| Number of reflections | 30114 | |
| <I/σ(I)> | 14 | 4.3 |
| Completeness [%] | 94.2 | 76.6 |
| Redundancy | 5.5 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293.15 | 200 mM sodium acetate 30% PEG 8,000 100 mM sodium cacodylate Protein was prepared in PBS |
