4WXX

The crystal structure of human DNMT1(351-1600)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	CCD
Collection date	2014-08-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9774
Spacegroup name	P 43
Unit cell lengths	138.568, 138.568, 221.536
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.215 - 2.622
R-factor	0.2069
Rwork	0.205
R-free	0.24530
RMSD bond length	0.003
RMSD bond angle	0.792
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.220
High resolution limit [Å]	2.620
Number of reflections	122755
<I/σ(I)>	12.8
Completeness [%]	98.6
Redundancy	5.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	289	45% PEG200, 50 mM CaCl2, 0.1 mM MES