4WWA
Crystal structure of binary complex Bud32-Cgi121
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-09-30 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.979180 |
Spacegroup name | I 41 |
Unit cell lengths | 111.540, 111.540, 86.420 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.200 - 2.953 |
R-factor | 0.221 |
Rwork | 0.217 |
R-free | 0.29590 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.057 |
Data reduction software | XDS (2011) |
Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.200 | 3.059 |
High resolution limit [Å] | 2.950 | 2.953 |
Number of reflections | 11205 | |
<I/σ(I)> | 14.32 | |
Completeness [%] | 99.8 | |
Redundancy | 4.58 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | 0.1 M Sodium Acetate pH 4.6, 2.0 M Ammounium Sulfate, 0.1 M NaCl and 10 mM Tris-HCl pH 7.5 |