4WV5
HEAT SHOCK PROTEIN 70 SUBSTRATE BINDING DOMAIN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-02-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 44.738, 52.597, 73.246 |
| Unit cell angles | 90.00, 95.77, 90.00 |
Refinement procedure
| Resolution | 21.540 - 2.040 |
| R-factor | 0.1887 |
| Rwork | 0.187 |
| R-free | 0.22390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wv7 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.650 | 2.150 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Rmerge | 0.037 | 0.130 |
| Number of reflections | 20760 | |
| <I/σ(I)> | 19.1 | 6.3 |
| Completeness [%] | 95.3 | 75.5 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 294 | 11 mg/ml protein in 25mM Tris-HCl, 150 mM NaCl, 1 mM TCEP was mixed 1:1 with reservior solution containing 0.1 M Sodium Acetate, pH 4.5, 2M Ammonium Sulfate |
