4WU3
Structure of the PTP-like myo-inositol phosphatase from Mitsuokella multacida in complex with myo-inositol-(1,3,4,5)-tetrakisphosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-04 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | .97934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 73.899, 86.680, 124.149 |
| Unit cell angles | 107.27, 91.69, 90.01 |
Refinement procedure
| Resolution | 44.540 - 2.200 |
| R-factor | 0.19014 |
| Rwork | 0.190 |
| R-free | 0.21341 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3f41 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.106 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.540 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.080 | 0.160 |
| Number of reflections | 146418 | |
| <I/σ(I)> | 6 | 4.3 |
| Completeness [%] | 98.8 | 96.6 |
| Redundancy | 2.2 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | PEG 8000, Polyethylene glycol, Tris chloride, beta-mercapto ethanol, glycerol |
