4WSD
Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site and with antibiotic paromomycin.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2013-06-29 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.400, 449.200, 621.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 255.475 - 2.950 |
Rwork | 0.193 |
R-free | 0.23490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3TVF 3TVE |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 300.000 | 3.050 | |
High resolution limit [Å] | 2.950 | 100.000 | 2.950 |
Rmerge | 0.300 | 0.031 | 4.193 |
Rmeas | 0.302 | 0.034 | 4.266 |
Total number of observations | 90425707 | ||
Number of reflections | 1213341 | 27 | 114461 |
<I/σ(I)> | 18.67 | 8.99 | 1.13 |
Completeness [%] | 99.9 | 58.7 | 99.8 |
Redundancy | 74.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |