4WRO
Complex of 70S ribosome with tRNA-Phe and mRNA with C-A mismatch in the second position in the A-site
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-04-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 209.400, 447.700, 619.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 151.956 - 3.050 |
| Rwork | 0.193 |
| R-free | 0.23070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3TVF 3TVE |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 200.000 | 3.150 | |
| High resolution limit [Å] | 3.050 | 100.000 | 3.050 |
| Rmerge | 0.325 | 0.029 | 4.566 |
| Rmeas | 0.327 | 0.034 | 4.629 |
| Total number of observations | 69639588 | ||
| Number of reflections | 1092032 | 11 | 100022 |
| <I/σ(I)> | 14.16 | 20.25 | 0.97 |
| Completeness [%] | 99.9 | 23.9 | 99.9 |
| Redundancy | 63.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | Tris-acetate, KSCN, PEG 550MME, PEG 20K |
