4WQT
Thermus thermophilus RNA polymerase complexed with an RNA cleavage stimulating factor (a GreA/Gfh1 chimeric protein)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-02-24 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 189.568, 263.770, 195.848 |
| Unit cell angles | 90.00, 116.83, 90.00 |
Refinement procedure
| Resolution | 45.790 - 4.400 |
| R-factor | 0.26 |
| Rwork | 0.260 |
| R-free | 0.31300 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS (1.3) |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.560 |
| High resolution limit [Å] | 4.400 | 4.400 |
| Rmerge | 0.669 | |
| Number of reflections | 104563 | |
| <I/σ(I)> | 7 | 1.5 |
| Completeness [%] | 97.0 | 95.1 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 293 | PEG 6000, potassium chloride, magnesium chloride, HEPES-NaOH |
