4WPI
Crystal structure of USP7 ubiquitin-like domains in extended conformation
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-04 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.97849 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 164.990, 164.990, 110.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.400 |
| R-factor | 0.2429 |
| Rwork | 0.241 |
| R-free | 0.28150 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.394 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 42.300 |
| High resolution limit [Å] | 3.400 |
| Number of reflections | 21462 |
| <I/σ(I)> | 24.3 |
| Completeness [%] | 99.7 |
| Redundancy | 9.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | sodium chloride, TRIS-HCl, 2-mercaptoethanol, betaine hydrochloride |
