4WPG
Group A Streptococcus GacA is an essential dTDP-4-dehydrorhamnose reductase (RmlD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-11-08 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.89 |
Spacegroup name | P 1 |
Unit cell lengths | 36.482, 45.977, 48.867 |
Unit cell angles | 66.30, 81.21, 95.27 |
Refinement procedure
Resolution | 43.770 - 1.100 |
R-factor | 0.1302 |
Rwork | 0.129 |
R-free | 0.15494 |
RMSD bond length | 0.022 |
RMSD bond angle | 2.073 |
Data reduction software | iMOSFLM |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.800 | 1.140 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 108172 | |
<I/σ(I)> | 22.5 | 5.7 |
Completeness [%] | 94.0 | 85.5 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.02 M of each carboxylic acid (0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M trisodium citrate, 0.2 M sodium potassium L-tartrate, 0.2 M sodium oxamate) and 0.1 M MES/imidazole pH 6.5 |