4WOL

Crystal Structure of the DAP12 transmembrane domain in lipidic cubic phase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	20.775, 35.709, 101.070
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.669 - 1.770
R-factor	0.1873
Rwork	0.183
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1afo
RMSD bond length	0.007
RMSD bond angle	0.900
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	33.670
High resolution limit [Å]	1.770
Rmerge	0.136
Number of reflections	7502
<I/σ(I)>	22.26
Completeness [%]	95.5
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		293	12.4% w/v PEG 3350, 0.149 M potassium thiocyanate