4WNO
Structure of ULK1 bound to an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-08-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.99995 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 66.780, 66.780, 116.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 57.904 - 1.560 |
| R-factor | 0.1799 |
| Rwork | 0.178 |
| R-free | 0.20760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4b6l |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.235 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.110 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmerge | 0.070 | 0.500 |
| Number of reflections | 38276 | |
| <I/σ(I)> | 18.1 | 2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.55 M sodium malonate pH 7.0, 0.35 M sodium malonate pH 5.0 |
