4WNC

Crystal structure of human wild-type GAPDH at 1.99 angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 12.3.1
Synchrotron site	ALS
Beamline	12.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-10-05
Detector	ADSC QUANTUM 315
Wavelength(s)	1.116
Spacegroup name	P 1 21 1
Unit cell lengths	132.537, 81.423, 147.802
Unit cell angles	90.00, 90.06, 90.00

Refinement procedure

Resolution	49.361 - 1.990
R-factor	0.139
Rwork	0.139
R-free	0.17900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u8f
RMSD bond length	0.009
RMSD bond angle	1.182
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.020
High resolution limit [Å]	1.990	5.400	1.990
Rmerge	0.092	0.040	0.336
Total number of observations	795088
Number of reflections	216430
<I/σ(I)>	19.1
Completeness [%]	99.9	99.4	99.9
Redundancy	3.7	3.7	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	20% (w/v) PEG6000, 0.1 M sodium acetate, 0.01 M ZnCl2