4WM6
High pressure protein crystallography of hen egg white lysozyme at 950 MPa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2013-02-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.71 |
| Spacegroup name | P 43 |
| Unit cell lengths | 107.269, 107.269, 36.891 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.760 - 1.850 |
| R-factor | 0.2035 |
| Rwork | 0.199 |
| R-free | 0.28445 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.728 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.850 |
| Number of reflections | 36471 |
| <I/σ(I)> | 19.1 |
| Completeness [%] | 99.3 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | Sodium chloride |
