4WLR

Crystal Structure of mUCH37-hRPN13 CTD-hUb complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-11-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.075
Spacegroup name	P 21 21 21
Unit cell lengths	60.120, 99.610, 99.909
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.422 - 1.997
R-factor	0.1828
Rwork	0.181
R-free	0.22730
RMSD bond length	0.008
RMSD bond angle	1.074
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX ((phenix.refine: dev_1760))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	38.420	38.420	2.050
High resolution limit [Å]	2.000	8.930	2.000
Rmerge	0.090	0.042	2.006
Rpim	0.024	0.012	0.556
Total number of observations	600550	6880	39852
Number of reflections	41390
<I/σ(I)>	17.7	58.7	1.5
Completeness [%]	99.7	99	96.2
Redundancy	14.5	12.7	13.7
CC(1/2)	0.999	0.998	0.589

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	277	25% PEG 3350, 220 mM MgCl2,100 mM Bis-Tris