4WJ3

Crystal structure of the asparagine transamidosome from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2013-01-23
Detector	RAYONIX MX225HE
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	93.140, 185.675, 290.359
Unit cell angles	90.00, 92.98, 90.00

Refinement procedure

Resolution	46.769 - 3.705
R-factor	0.2941
Rwork	0.292
R-free	0.32860
RMSD bond length	0.003
RMSD bond angle	0.836
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall
Low resolution limit [Å]	48.120
High resolution limit [Å]	3.700
Number of reflections	96512
<I/σ(I)>	6.36
Completeness [%]	92.1
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	298	MES, lithium sulfate, PEG3350, hexammine cobalt(III) chloride