4WIQ

The structure of Murine alpha-Dystroglycan T190M mutant N-terminal domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-05-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	H 3
Unit cell lengths	71.879, 71.879, 144.296
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.099 - 1.590
R-factor	0.147
Rwork	0.146
R-free	0.16320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uc2
RMSD bond length	0.010
RMSD bond angle	1.245
Data reduction software	XDS (january 10, 2014)
Data scaling software	Aimless (0.2.17)
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.100	1.650
High resolution limit [Å]	1.590	1.590
Rmerge	0.045	0.442
Number of reflections	35504
<I/σ(I)>	15.28	1.74
Completeness [%]	95.0	71.8
Redundancy	3.4	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	Protein solution: 5.25 mg/mL in 25 mM Tris, 150 mM NaCl and 2.5 % glycerol; pH 7.5 Precipitant solution: 0.8 M citrate buffer; pH 7.0 Drops volume: 1 microL protein solution + 1 microL precipitant solution Reservoir volume: 1 mL Crystals appeared after few days