4WHA
Lipoxygenase-1 (soybean) L546A/L754A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-03-07 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.116 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 94.126, 92.644, 49.572 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 66.026 - 1.700 |
| R-factor | 0.1384 |
| Rwork | 0.137 |
| R-free | 0.17890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3pzw |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.891 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.030 | 1.761 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.700 | |
| Number of reflections | 28667 | |
| <I/σ(I)> | 10.09 | 1.6 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 5.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 290 | 9% PEG3350, 200 mM sodium acetate, pH 5.5 |
