4WFG
Human TRAAK K+ channel in a Tl+ bound conductive conformation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-04-19 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9781 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.798, 138.760, 96.317 |
| Unit cell angles | 90.00, 94.52, 90.00 |
Refinement procedure
| Resolution | 48.000 - 3.000 |
| R-factor | 0.20395 |
| Rwork | 0.202 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4i9w |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.898 |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.050 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 40099 | |
| <I/σ(I)> | 14.7 | 0.9 |
| Completeness [%] | 99.6 | 99.7 |
| Redundancy | 5.6 | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 277 | 50 MM TRIS PH 8.8, 200 MM CA(NO3)2, 27-30%(VOL/VOL) PEG400, 52 MM TRICHLOROETHANOL |
