4WD8

Crystal structure of a bacterial Bestrophin homolog from Klebsiella pneumoniae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-06-25
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	114.079, 160.034, 161.881
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.961 - 2.300
R-factor	0.1971
Rwork	0.196
R-free	0.21450
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.955
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall
Low resolution limit [Å]	40.000
High resolution limit [Å]	2.300
Number of reflections	131752
<I/σ(I)>	11.8
Completeness [%]	99.9
Redundancy	12.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	0.05 M zinc acetate, 6% v/v ethylene glycol, 0.1 M sodium cacodylate, pH 6.0, 6.6 % w/v PEG 8000