4WC4

tRNA-processing enzyme complex 2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	95
Detector technology	CCD
Collection date	2014-06-18
Detector	ADSC QUANTUM 270
Wavelength(s)	0.98
Spacegroup name	P 21 21 2
Unit cell lengths	59.310, 111.760, 127.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.881 - 3.501
R-factor	0.2782
Rwork	0.277
R-free	0.30200
RMSD bond length	0.002
RMSD bond angle	0.492
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	PHENIX ((phenix.refine: 1.9_1692))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000		3.590
High resolution limit [Å]	3.500	15.660	3.500
Rmerge	0.222	0.039	1.309
Rmeas	0.239	0.043	1.410
Total number of observations	80315
Number of reflections	11158	74	797
<I/σ(I)>	8.94	31.27	1.86
Completeness [%]	99.2	47.4	99.4
Redundancy	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	isopropanol, potassium chloride, magnesium chloride, sodium cacodylate