4WBO
Bovine G Protein Coupled Receptor Kinase 1 in Complex with Amlexanox
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-23 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9787 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 118.112, 119.166, 174.261 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.890 - 2.810 |
| R-factor | 0.24277 |
| Rwork | 0.241 |
| R-free | 0.26726 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.919 |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.870 |
| High resolution limit [Å] | 2.820 | 2.820 |
| Rmerge | 0.097 | |
| Number of reflections | 60016 | |
| <I/σ(I)> | 19.5 | 1.4 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 7.4 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.25 | 277 | 500 mM NaCl, 100 mM MES pH 6.25, and 20% PEG3350 |
