4WB6
Crystal structure of a L205R mutant of human cAMP-dependent protein kinase A (catalytic alpha subunit)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-04-25 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.169, 90.193, 90.377 |
| Unit cell angles | 90.00, 96.12, 90.00 |
Refinement procedure
| Resolution | 40.492 - 2.100 |
| R-factor | 0.1903 |
| Rwork | 0.188 |
| R-free | 0.23710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wb8 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.057 |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.064 | 0.850 |
| Number of reflections | 52562 | |
| <I/σ(I)> | 9 | |
| Completeness [%] | 99.8 | 99.3 |
| Redundancy | 3.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | 10% PEG 8000, 0.1M HEPES pH 7.5 |
