4WA8
Methanopyrus Kandleri FEN-1 nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-22 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.331, 86.193, 147.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.430 - 2.200 |
| R-factor | 0.20165 |
| Rwork | 0.200 |
| R-free | 0.23134 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rxw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.194 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.430 | 2.260 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.088 | 0.741 |
| Number of reflections | 35363 | |
| <I/σ(I)> | 10.6 | 2.2 |
| Completeness [%] | 96.4 | 88.5 |
| Redundancy | 5.9 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 290 | PEG4000, Tris-HCL, sodium chloride, calcium chloride |
