4WA6
Structure of yeast SAGA DUBm with Sgf73 N59D mutant at 2.36 angstroms resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-06-28 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.034 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.076, 68.004, 137.858 |
| Unit cell angles | 90.00, 106.75, 90.00 |
Refinement procedure
| Resolution | 47.410 - 2.360 |
| R-factor | 0.18615 |
| Rwork | 0.183 |
| R-free | 0.24380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.654 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 47.410 |
| High resolution limit [Å] | 2.360 |
| Number of reflections | 59279 |
| <I/σ(I)> | 23 |
| Completeness [%] | 100.0 |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 100mM Bis Tris, 18% PEG3350, 100mM Ammonium Sulfate |
