4W2G

Crystal structure of the Thermus thermophilus 70S ribosome in complex with pactamycin (soaked), mRNA and three deacylated tRNAs in the A, P and E sites

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-12-09
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97900
Spacegroup name	P 21 21 21
Unit cell lengths	209.350, 449.010, 621.980
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	145.516 - 2.550
R-factor	0.2276
Rwork	0.225
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.033
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	310.990		2.620
High resolution limit [Å]	2.550	11.400	2.550
Rmerge	0.139	0.012	0.012
Rmeas		0.033	1.480
Number of reflections	1857656	21042	137425
<I/σ(I)>	7.61	26.63	1
Completeness [%]	99.0	95	99.4
Redundancy	3.723

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME, VAPOR DIFFUSION, temperature 292K