4V9C

Allosteric control of the ribosome by small-molecule antibiotics

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-06-13
Detector	ADSC QUANTUM 315
Spacegroup name	P 21 21 21
Unit cell lengths	212.180, 433.900, 608.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	70.000 - 3.300
R-factor	0.206
Rwork	0.206
R-free	0.25500
Structure solution method	FOURIER SYNTHESIS
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]		3.390
High resolution limit [Å]	3.300	3.300
Rmerge	0.182	0.868
Number of reflections	793808
<I/σ(I)>	5.76	1.12
Completeness [%]	95.2	86.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5	291	PEG800, MPD, pH 6.5, microbatch, temperature 291K
1		6.5	291	PEG800, MPD, pH 6.5, microbatch, temperature 291K