4V9A
Crystal Structure of the 70S ribosome with tetracycline.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-02-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 209.270, 448.540, 615.750 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 173.073 - 3.300 |
R-factor | 0.2026 |
Rwork | 0.202 |
R-free | 0.25390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3UZ6 3UZ7 3UZ8 3UZ9 |
RMSD bond length | 0.005 |
RMSD bond angle | 0.682 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX (dev_987) |
Data quality characteristics
Overall | Inner shell | |
Low resolution limit [Å] | 300.000 | 300.000 |
High resolution limit [Å] | 3.300 | 200.000 |
Rmerge | 0.423 | 0.122 |
Number of reflections | 859965 | 2 |
<I/σ(I)> | 10.71 | |
Completeness [%] | 100.0 | 66.7 |
Redundancy | 45.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4% PEG20K, 4% PEGMME 550, 100mM Tris/HAc, 200mM KSCN, pH 7.5, vapor diffusion, hanging drop, temperature 293K |