4V8P
T.thermophila 60S ribosomal subunit in complex with initiation factor 6.
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-08-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 320.190, 289.250, 535.040 |
| Unit cell angles | 90.00, 91.22, 90.00 |
Refinement procedure
| Resolution | 19.998 - 3.520 |
| R-factor | 0.2163 |
| Rwork | 0.216 |
| R-free | 0.24450 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1jj2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.144 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 3.720 |
| High resolution limit [Å] | 3.520 | 3.520 |
| Rmerge | 0.130 | 0.810 |
| Number of reflections | 1192534 | |
| <I/σ(I)> | 8.8 | 1.6 |
| Completeness [%] | 99.3 | 99.6 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
