4V6B
Crystal structure of human ferritin Phe167SerfsX26 mutant.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-07-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9800 |
Spacegroup name | P 1 |
Unit cell lengths | 212.340, 236.940, 249.710 |
Unit cell angles | 94.69, 115.06, 114.96 |
Refinement procedure
Resolution | 50.000 - 2.850 |
Rwork | 0.249 |
R-free | 0.29100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2fg8 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.950 |
High resolution limit [Å] | 2.850 | 2.850 |
Rmerge | 0.080 | 0.322 |
Number of reflections | 846572 | |
<I/σ(I)> | 9.653 | 1.91 |
Completeness [%] | 94.2 | 62.9 |
Redundancy | 2.4 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 100 mM MES pH 6.5, 5% w/v PEG 6000, 2% v/v Ethylene glycol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |