4V5F
The structure of the ribosome with elongation factor G trapped in the post-translocational state
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 291.839, 270.358, 402.363 |
| Unit cell angles | 90.00, 91.73, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.600 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2J00 2J01 2J02 2J03. |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.200 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.700 |
| High resolution limit [Å] | 3.600 | 3.600 |
| Rmerge | 0.370 | 1.150 |
| Number of reflections | 690340 | |
| <I/σ(I)> | 6.64 | 1.54 |
| Completeness [%] | 96.0 | 89.9 |
| Redundancy | 9.2 | 6.91 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 100 MM MES PH 6.5, 12%PEG 20K, 50 MM KCL, 10 MM NH4CL, 3UM DEOXY-BIG-CHAP |
