4V51
Structure of the Thermus thermophilus 70S ribosome complexed with mRNA, tRNA and paromomycin
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-02-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 213.318, 452.953, 631.356 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.800 |
| R-factor | 0.2715 |
| Rwork | 0.272 |
| R-free | 0.31330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1YL4 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.260 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.280 | 0.750 |
| Number of reflections | 1342659 | |
| <I/σ(I)> | 7.3 | 2.1 |
| Completeness [%] | 91.8 | 72 |
| Redundancy | 9.1 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.2M KSCN, 4% PEG 20K, 4% PEG550 MME, 0.1M TRIS-ACETATE PH 7 |
