4V2Z
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with Pomalidomide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-26 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.469, 60.144, 88.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.350 - 1.450 |
| R-factor | 0.16961 |
| Rwork | 0.168 |
| R-free | 0.20254 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.203 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 1.540 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.040 | 0.450 |
| Number of reflections | 54054 | |
| <I/σ(I)> | 16.32 | 2.45 |
| Completeness [%] | 99.5 | 99.1 |
| Redundancy | 3.4 | 3.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1 M SODIUM ACETATE PH 4.6, 15 %(W/V) PEG 20000 |
