4V2R
Ironing out their differences: Dissecting the structural determinants of a phenylalanine aminomutase and ammonia lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 77 |
| Detector technology | PIXEL |
| Collection date | 2013-02-23 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 181.485, 76.486, 120.419 |
| Unit cell angles | 90.00, 120.02, 90.00 |
Refinement procedure
| Resolution | 104.260 - 2.200 |
| R-factor | 0.21349 |
| Rwork | 0.212 |
| R-free | 0.24746 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2yii |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.362 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.070 | 0.400 |
| Number of reflections | 72574 | |
| <I/σ(I)> | 12 | 3 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
