4V0P
Crystal structure of the MAGE homology domain of human MAGE-A3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-29 |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 61.473, 61.473, 292.831 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.394 - 2.070 |
| R-factor | 0.202 |
| Rwork | 0.200 |
| R-free | 0.23810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wa0 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.761 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.200 | 2.130 |
| High resolution limit [Å] | 2.070 | 2.070 |
| Rmerge | 0.060 | 1.110 |
| Number of reflections | 21270 | |
| <I/σ(I)> | 19.9 | 2.2 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.2 | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M MG FORMATE, 15% PEG 3350 |
