4UZB

KSHV LANA (ORF73) C-terminal domain mutant bound to LBS1 DNA (R1039Q, R1040Q, K1055E, K1109A, D1110A, A1121E, K1138S, K1140D, K1141D)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, DESY BEAMLINE P11
Synchrotron site	PETRA III, DESY
Beamline	P11
Temperature [K]	293
Detector technology	PIXEL
Collection date	2013-12-05
Detector	DECTRIS PILATUS 6M
Spacegroup name	I 21 3
Unit cell lengths	177.840, 177.840, 177.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.530 - 2.865
R-factor	0.1234
Rwork	0.122
R-free	0.15470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ypy
RMSD bond length	0.008
RMSD bond angle	1.059
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (PHASER-MR)
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.530	3.040
High resolution limit [Å]	2.870	2.870
Rmerge	0.080	0.860
Number of reflections	21605
<I/σ(I)>	15.65	1.99
Completeness [%]	99.9	99.7
Redundancy	6.4	6.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.6	297	0.9 UL OF 1.60 MM LANA(1008-1146) MUTANT, 0.96 MM DSDNA, 10 MM BISTRIS PH 6.5, 0.9 M NACL, AND 5 MM DTT WERE ADDED TO 0.9 UL OF RESERVOIR SOLUTION CONTAINING 50 MM MES PH 6.6, 27% PEG4000, AND 0.3 M AMMONIUM ACETATE IN A HANGING DROP SETUP. CRYSTALS GREW WITHIN FIVE DAYS AT 24 DEGREES CENTIGRADE.