4UY2
Crystal structure of the complex of the extracellular domain of human alpha9 nAChR with alpha-bungarotoxin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-08-30 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.319, 119.348, 79.107 |
| Unit cell angles | 90.00, 103.02, 90.00 |
Refinement procedure
| Resolution | 47.185 - 2.697 |
| R-factor | 0.2617 |
| Rwork | 0.258 |
| R-free | 0.33120 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1D01 2qc1 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.425 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.110 | 1.450 |
| Number of reflections | 21021 | |
| <I/σ(I)> | 9.9 | 1 |
| Completeness [%] | 99.5 | 97 |
| Redundancy | 5.1 | 5.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.1 M MES PH 6.0, 0.2 M NACL, 30% W/V JEFFAMINE ED2003 |
