4UXU
Crystal Structure of the Extracellular Domain of the Human Alpha9 Nicotinic Acetylcholine Receptor In Complex with Methyllycaconitine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-05-25 |
| Detector | DECTRIS PILATUS 2M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.434, 66.246, 75.191 |
| Unit cell angles | 90.00, 102.76, 90.00 |
Refinement procedure
| Resolution | 41.606 - 1.710 |
| R-factor | 0.2112 |
| Rwork | 0.206 |
| R-free | 0.23460 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d01 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.344 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.600 | 1.770 |
| High resolution limit [Å] | 1.710 | 1.710 |
| Rmerge | 0.090 | 0.840 |
| Number of reflections | 63697 | |
| <I/σ(I)> | 6.9 | 1.4 |
| Completeness [%] | 97.9 | 94 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES PH 7.5, 15% PEG 20000, 7.5% ETHANOL |
