4UWC
Fibroblast growth factor receptor 1 kinase in complex with JK-P3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-09-19 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 207.464, 58.422, 65.946 |
| Unit cell angles | 90.00, 107.38, 90.00 |
Refinement procedure
| Resolution | 29.210 - 1.960 |
| R-factor | 0.1789 |
| Rwork | 0.177 |
| R-free | 0.20580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | INTERNAL FGFR1 MODEL |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.030 |
| Data reduction software | XDS (VERSION MAY 10 2010) |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.210 | 2.010 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.030 | 0.560 |
| Number of reflections | 47605 | |
| <I/σ(I)> | 16.8 | 1.5 |
| Completeness [%] | 87.8 | 41.1 |
| Redundancy | 3.4 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG8000, 20% ETHYLENE GLYCOL, 0.2M AMMONIUM SULPHATE, 0.1M PCTP PH 6.75 |
